Prep HPLC Column, Diamond Hydride, 4 um, 100 A. 21.2 mm ID x 150 mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 70000‑P21‑150 Diamond Hydride Preparative HPLC Column is engineered for resolution‑critical preparative separations where shorter preparative columns do not provide adequate separation for reliable purification. This extended‑bed preparative format increases resolving power while maintaining practical throughput for high‑load workflows.
Preparative Use Case: Why 21.2 mm ID × 150 mm
The 21.2 mm internal diameter classifies this column as fully preparative, supporting milligram‑ to gram‑scale purification in laboratories operating dedicated preparative HPLC systems.
The 150 mm column length provides a significant increase in chromatographic performance:
Higher theoretical plate count than 50 mm and 100 mm prep columns
Improved resolution of closely eluting or structurally similar compounds
Greater peak capacity for complex polar mixtures
Increased confidence during high‑value fraction collection
This configuration is typically selected when resolution is the dominant requirement, but a full 250 mm preparative column is unnecessary or impractical.
Diamond Hydride™ TYPE‑C Stationary Phase Advantages
Manufactured using Cogent TYPE‑C™ silica hydride technology, the Diamond Hydride stationary phase delivers robust performance advantages for preparative HILIC separations:
Highly reproducible retention of polar and basic analytes
Faster equilibration compared to conventional bonded‑silica HILIC phases
Excellent chemical stability under high‑organic mobile‑phase conditions
Long column lifetime in demanding preparative environments
This chemistry is particularly effective for purification of:
Peptides and peptide‑like compounds
Metabolites and highly polar small molecules
Amine‑containing and basic analytes
Compounds best isolated using HILIC‑based retention mechanisms
Purpose‑Built for Resolution‑Focused Preparative Work
The 70000‑P21‑150 enables predictable scale‑up from analytical and semi‑preparative Diamond Hydride methods while preserving chromatographic behavior. It is frequently used in method‑critical purification, process development, and regulated workflows where separation quality cannot be compromised.
System Requirements
Typical operating flow rates: ~20–35 mL/min
This column requires a preparative HPLC system capable of:
Sustained high‑flow solvent delivery
Large injection volumes
Pressure limits compatible with longer preparative columns
Appropriate detector configuration and fraction collection
Standard analytical or semi‑preparative HPLC systems are not suitable for 21.2 mm ID preparative columns.
Flow rates should be optimized based on mobile‑phase composition, viscosity, pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
These columns represent a significant investment and are often application‑specific. To ensure customers receive the correct column for their workflow:
Pricing and technical support are provided through our Customer Service Department via formal quotation
Columns are produced on order, based on customer requirements
Orders cannot be canceled or returned once placed
Learn more about Custom Orders and our Custom Order policies.